Primer
Part:BBa_K1456012:Design
Designed by: Mustafa Yılmaz Group: iGEM14_ATOMS-Turkiye (2014-08-13)
Human Tissue Plasminogen Activator reverse primer with restriction site, 2 stop codons and 6X His
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 14
Illegal PstI site found at 2 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 14
Illegal PstI site found at 2 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 8
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 14
Illegal PstI site found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 14
Illegal PstI site found at 2 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
none
Source
none